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- BOSTER
- A00745-1-50ul
- 中国
- 2025年07月13日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00745-1 |
|---|---|
| 产品名称 | Anti-CCNB1 Antibody |
| 基因名 | CCNB1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 55KD |
| 免疫原 | E.coli-derived human Cyclin B1/CCNB1 recombinant protein (Position: M1-L383). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing. |
| 背景资料 | G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. It is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. |
| 研究类别 | 1. Matsuo, T., Yamaguchi, S., Mitsui, S., Emi, A., Shimoda, F., Okamura, H. Control mechanism of the circadian clock for timing of cell division in vivo. Science 302: 255-259, 2003.2. Milatovich, A., Francke, U. Human cyclin B1 gene (CCNB1) assigned to chromosome 5 (q13-qter). Somat. Cell Molec. Genet. 18: 303-307, 1992.3. Moore, J. D., Kirk, J. A., Hunt, T. Unmasking the S-phase-promoting potential of cyclin B1. Science 300: 987-990, 2003. |
| Uniprot ID | CCNB1: P14635 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti-CCNB1 antibody (A00745-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Human HELA whole cell lysates,
Lane 2: Human HEK293 whole cell lysates,
Lane 3: Human Jurkat whole cell lysates,
Lane 4: Human Raji whole cell lysates,
Lane 5: Human K562 whole cell lysates,
Lane 6: Human U2OS whole cell lysates,
Lane 7: Human CACO-2 whole cell lysates,
Use rabbit anti- CCNB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.|Figure 2. Western blot analysis of anti-CCNB1 antibody (A00745-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat spleen tissue lysates,
Lane 2: Mouse thymus tissue lysates,
Lane 3: Mouse NIH/3T3 whole cell lysates.
Use rabbit anti- CCNB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.|Figure 3.IHC analysis using anti-CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4.IHC analysis using anti-CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human tonsil cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5.IHC analysis using anti-CCNB1 antibody (A00745-1). detected in paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis using anti- CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human seminoma testis tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human endometrial adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 9.ICC analysis using anti-CCNB1 antibody (A00745-1) was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).|Figure 10.Flow cytometry analysis of A431 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Human HELA whole cell lysates,
Lane 2: Human HEK293 whole cell lysates,
Lane 3: Human Jurkat whole cell lysates,
Lane 4: Human Raji whole cell lysates,
Lane 5: Human K562 whole cell lysates,
Lane 6: Human U2OS whole cell lysates,
Lane 7: Human CACO-2 whole cell lysates,
Use rabbit anti- CCNB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.|Figure 2. Western blot analysis of anti-CCNB1 antibody (A00745-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat spleen tissue lysates,
Lane 2: Mouse thymus tissue lysates,
Lane 3: Mouse NIH/3T3 whole cell lysates.
Use rabbit anti- CCNB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.|Figure 3.IHC analysis using anti-CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4.IHC analysis using anti-CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human tonsil cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5.IHC analysis using anti-CCNB1 antibody (A00745-1). detected in paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis using anti- CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human seminoma testis tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- CCNB1 antibody (A00745-1). detected in paraffin-embedded section of human endometrial adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 9.ICC analysis using anti-CCNB1 antibody (A00745-1) was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).|Figure 10.Flow cytometry analysis of A431 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Cyclin B1/CCNB1 Antibody(A00745-1-50ul)
￥1180
