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大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | MA00050 |
|---|---|
| 产品名称 | Anti-EZH2 Antibody (Clone#OTI2B10) |
| 基因名 | EZH2 |
| 抗体来源 | Mouse |
| 克隆 | OTI2B10 |
| 抗体亚型 | Mouse IgG1 |
| 分子量 | 85.8KD |
| 免疫原 | Human recombinant protein fragment corresponding to amino acids 182-431 of human EZH2 ( NP_004447) produced in E.coli. |
| 内容 | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| 纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
| 背景资料 | Enhancer of zeste homolog 2 (EZH2) is a histone-lysine N-methyltransferase enzyme encoded by EZH2 gene. It is mapped to 7q36.1. This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. |
| 研究类别 | 1. Kim, Kimberly; Roberts, Charles (18 December 2015). "Targeting EZH2 in cancer". Nature Medicine. 22: 128–134.2. Viré E, Brenner C, Deplus R, Blanchon L, Fraga M, Didelot C, et al. (2006). "The Polycomb group protein EZH2 directly controls DNA methylation". Nature. 439 (7078): 871–4. |
| Uniprot ID | EZH2: Q15910 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:150 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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文献和实验Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
a simple ELISA method to detect human anti-antibody response.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
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