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Anti-EZH2 Antibody (Clone#OTI2

B10)(MA00050-50ul)
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  • ¥1180
  • BOSTER已认证
  • MA00050-50ul
  • 中国
  • 2025年07月08日
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    • 库存

      大量

    • 供应商

      广州威佳科技有限公司

    • 规格

      50ul

    产品概况

    货号 MA00050
    产品名称 Anti-EZH2 Antibody (Clone#OTI2B10)
    基因名 EZH2
    抗体来源 Mouse
    克隆 OTI2B10
    抗体亚型 Mouse IgG1
    分子量 85.8KD
    免疫原 Human recombinant protein fragment corresponding to amino acids 182-431 of human EZH2 ( NP_004447) produced in E.coli.
    内容 PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
    纯化方式 Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
    浓度 500 ug/ml
    产品形态 Liquid
    保存条件 Stable for 12 months from date of receipt. Store at -20°C as received.
    背景资料 Enhancer of zeste homolog 2 (EZH2) is a histone-lysine N-methyltransferase enzyme encoded by EZH2 gene. It is mapped to 7q36.1. This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
    研究类别 1. Kim, Kimberly; Roberts, Charles (18 December 2015). "Targeting EZH2 in cancer". Nature Medicine. 22: 128–134.2. Viré E, Brenner C, Deplus R, Blanchon L, Fraga M, Didelot C, et al. (2006). "The Polycomb group protein EZH2 directly controls DNA methylation". Nature. 439 (7078): 871–4.
    Uniprot ID EZH2: Q15910

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    [list_product_images]Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and EZH2-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-EZH2 monoclonal antibody MA00050, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control.|Figure 2. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-EZH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, MA00050)|Figure 3. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-EZH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, MA00050)|Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EZH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, MA00050)|Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EZH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EZH2. [/list_product_images]

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