- ￥1960
- BOSTER
- A15467-2-100ul
- 中国
- 2025年07月12日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A15467-2 |
|---|---|
| 产品名称 | Anti-SLC5A4 Antibody |
| 基因名 | SLC5A4 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 80KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human SLC5A4 (EDYPEKSRGCLKKAYDLFCGLQK). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The low affinity sodium-glucose cotransporter also known as the sodium/glucose cotransporter 3 (SGLT3) or solute carrier family 5 member 4 (SLC5A4) is a protein that in humans is encoded by the SLC5A4 gene. Predicted to enable glucose:sodium symporter activity and proton transmembrane transporter activity. Predicted to be involved in sodium ion transport. Predicted to act upstream of or within proton transmembrane transport. Predicted to be active in plasma membrane. Predicted to be integral component of membrane. |
| 研究类别 | 1. Bianchi, L., Diez-Sampedro, A. A single amino acid change converts the sugar sensor SGLT3 into a sugar transporter. PLoS One 5: e10241, 2010. Note: Electronic Article. 2. Kothinti, R. K., Blodgett, A. B., North, P. E., Roman, R. J., Tabatabai, N. M. A novel SGLT is expressed in the human kidney. Europ. J. Pharm. 690: 77-83, 2012.3. Stumpf, A. M. Personal Communication. Baltimore, Md. 10/21/2019. |
| Uniprot ID | SLC5A4: Q9NY91 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- SLC5A4 antibody (A15467-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human 293T whole cell lysates.
Use rabbit anti- SLC5A4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SLC5A4 at approximately 80KD. The expected band size for SLC5A4 is at 72KD.|Figure 2. Flow cytometry analysis of 293T cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human 293T whole cell lysates.
Use rabbit anti- SLC5A4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SLC5A4 at approximately 80KD. The expected band size for SLC5A4 is at 72KD.|Figure 2. Flow cytometry analysis of 293T cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SLC5A4 Antibody(A15467-2-100ul)
￥1960
