相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | BA3763 |
|---|---|
| 产品名称 | Anti-SSR3 Antibody |
| 基因名 | SSR3 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 21KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3(8-23aa KQQSEEDLLLQDFSRN), identical to the related rat and mouse sequences. |
| 内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | SSR3(signal sequence receptor, gamma) is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al.(1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross(2012)mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence(GRCh37). Hartmann et al.(1993)found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes. |
| 研究类别 | 1. Gross, M. B.Personal Communication.Baltimore, Md. 6/20/2012.2. Hartmann, E., Gorlich, D., Kostka, S., Otto, A., Kraft, R., Knespel, S., Burger, E., Rapoport, T. A., Prehn, S.A tetrameric complex of membrane proteins in the endoplasmic reticulum.Europ. J. Biochem. 214: 375-381, 1993. |
| Uniprot ID | SSR3: Q9UNL2 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of anti-SSR3 antibody (BA3763). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SSR3 antigen affinity purified polyclonal antibody (BA3763) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SSR3 at approximately 21 kDa. The expected band size for SSR3 is at 21 kDa.|Figure 2. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 5. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of mouse brain cerebral cortex tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 6. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of mouse brain hippocampus tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 7. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of rat brain cerebral cortex tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 8. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of rat brain hippocampus tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 9. ICC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in an immunocytochemical section of Hela cells. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 10. IF analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of mouse brain tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 11. IF analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of rat brain tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 12. IF analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of human placenta tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). [/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SSR3 antigen affinity purified polyclonal antibody (BA3763) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SSR3 at approximately 21 kDa. The expected band size for SSR3 is at 21 kDa.|Figure 2. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 5. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of mouse brain cerebral cortex tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 6. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of mouse brain hippocampus tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 7. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of rat brain cerebral cortex tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 8. IHC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of rat brain hippocampus tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 9. ICC analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in an immunocytochemical section of Hela cells. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 10. IF analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of mouse brain tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 11. IF analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of rat brain tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 12. IF analysis of SSR3 using anti-SSR3 antibody (BA3763).
SSR3 was detected in a paraffin-embedded section of human placenta tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). [/list_product_images]
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Anti-SSR3 Antibody(BA3763-100ul)
¥1960








