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- M03148-100ul
- 中国
- 2025年07月13日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | M03148 |
|---|---|
| 产品名称 | Anti-BCAM Antibody |
| 基因名 | BCAM |
| 抗体来源 | Rabbit |
| 克隆 | Monoclonal(Clone:22B46) |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 85KD |
| 免疫原 | A synthesized peptide derived from human CD239 |
| 内容 | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.5mg/ml BSA. |
| 纯化方式 | Affinity-chromatography |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Basal cell adhesion molecule, also known as Lutheran antigen, is a plasma membraneglycoprotein that in humans is encoded by the BCAM gene. It is mapped to 19q13.32. This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Campbell, I. G., Foulkes, W. D., Senger, G., Trowsdale, J., Garin-Chesa, P., Rettig, W. J. Molecular cloning of the B-CAM cell surface glycoprotein of epithelial cancers: a novel member of the immunoglobulin superfamily. Cancer Res. 54: 5761-5765, 1994.2. Cook, P. J. L. The Lutheran-secretor recombination fraction in man: a possible sex difference. Ann. Hum. Genet. 28: 393-401, 1965.3. Daniels, G. L., Le Pennec, P. Y., Rouger, P., Salmon, C., Tippett, P. The red cell antigens Au(a) and Au(b) belong to the Lutheran system. Vox Sang. 60: 191-192, 1991. |
| Uniprot ID | BCAM: P50895 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| IHC: | 1:50-1:200 |
| IP: | 1:50 |
| FCM: | 1:50 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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文献和实验Purification Of Anti Peptide Antibody
a parastaltic pump and recirculate the serum ON or just batch bind the serum ON. Wash with 5 col vols TBS. Wash with 10 col vols 0.5 M NaCl, 20mM TrisCl pH 7.4, 0.2 Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine
a simple ELISA method to detect human anti-antibody response.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
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