- ￥1180
- BOSTER
- A01940-50ul
- 中国
- 2025年06月24日
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广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01940 |
|---|---|
| 产品名称 | Anti-PRKCB Antibody |
| 基因名 | PRKCB |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 77KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human PKC beta 1/PRKCB(NFDKEFTRQPVELTPTDKLFIMNLDQNE). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Protein kinase C beta type is an enzyme that in humans is encoded by the PRKCB gene. It is a member of the protein kinase C (PKC) gene family. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. It has been found that PRKCB activated by oxidative conditions in the cell, induces phosphorylation of p66(SHC) and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase PIN1. |
| 研究类别 | 1. "Entrez Gene: PRKCB1 protein kinase C, beta 1".2. Pinton, P., Rimessi, A., Marchi, S., Orsini, F., Migliaccio, E., Giorgio, M., Contursi, C., Minucci, S., Mantovani, F., Wieckowski, M. R., Del Sal, G., Pelicci, P. G., Rizzuto, R. Protein kinase C-beta and prolyl isomerase 1 regulate mitochondrial effects of the life-span determinant p66(Shc)Science 315: 659-663, 2007. |
| Uniprot ID | PRKCB: P05771 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940). Lane 1: human K562 whole cell lysate,Lane 2: human THP-1 whole cell lysate,Lane 3: human HEK293 whole cell lysate,Lane 4: human Jurkat whole cell lysate,Lane 5: human Raji whole cell lysate,Lane 6: human HL-60 whole cell lysate,Lane 7: human A549 whole cell lysate. anti-PKC beta 1 antigen affinity purified polyclonal antibody (Catalog # A01940)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for PKC beta 1 at approximately 77KD. The expected band size for PKC beta 1 is at 77KD.|Figure 2. Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940). Lane 1: rat thymus tissue lysate,Lane 2: rat spleen tissue lysate,Lane 3: rat brain tissue lysate,Lane 4: rat C6 whole cell lysate,Lane 5: mouse thymus tissue lysate,Lane 6: mouse spleen tissue lysate,Lane 7: mouse brain tissue lysate,Lane 8: mouse Neuro-2a whole cell lysate,Lane 9: mouse NIH3T3 whole cell lysate. anti-PKC beta 1 antigen affinity purified polyclonal antibody (Catalog # A01940)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for PKC beta 1 at approximately 77KD. The expected band size for PKC beta 1 is at 77KD.|Figure 3. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of mouse small intestine tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of rat lung tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human intestinal cancer tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human prostatic cancer tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 7. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human placenta tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
PKC beta 1 was detected in paraffin-embedded section of mouse small intestine tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of rat lung tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human intestinal cancer tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human prostatic cancer tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 7. IHC analysis of PKC beta 1 using anti-PKC beta 1 antibody (A01940).
PKC beta 1 was detected in paraffin-embedded section of human placenta tissues. anti-PKC beta 1 Antibody (A01940) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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Anti-PKC beta 1/PRKCB Antibody(A01940-50ul)
￥1180
