- ￥1960
- BOSTER
- A02643-1-100ul
- 中国
- 2025年07月12日
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产品概况
| 货号 | A02643-1 |
|---|---|
| 产品名称 | Anti-GGCX Antibody |
| 基因名 | GGCX |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 88KD |
| 免疫原 | E.coli-derived human GGCX recombinant protein (Position: M75-A730). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Gamma-glutamyl carboxylase is an enzyme that in humans is encoded by the GGCX gene, located on chromosome 2 at 2p12. This gene encodes an integral membrane protein of the rough endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma carboxyl glutamate, a modification that is required for their activity. The vitamin K-dependent protein substrates have a propeptide that binds the enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting as co-substrates. Vitamin K-dependent proteins affect a number of physiologic processes including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants of this gene have been associated with pseudoxanthoma elasticum-like disorder with associated multiple coagulation factor deficiency. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Brenner, B., Sanchez-Vega, B., Wu, S.-M., Lanir, N., Stafford, D. W., Solera, J. A missense mutation in a gamma-glutamyl carboxylase gene causes combined deficiency of all vitamin K-dependent blood coagulation factors. Blood 92: 4554-4559, 1998. 2. Brenner, B., Tavori, S., Zivelin, A., Keller, C. B., Suttie, J. W., Tatarsky, I., Seligsohn, U. Hereditary deficiency of all vitamin K-dependent procoagulants and anticoagulants. Brit. J. Haemat. 75: 537-542, 1990.3. Kuo, W.-L., Stafford, D. W., Cruces, J., Gray, J., Solera, J. Chromosomal localization of the gamma-glutamyl carboxylase gene at 2p12. Genomics 25: 746-748, 1995. |
| Uniprot ID | GGCX: P38435 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- GGCX Antibody (A02643-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: HEK293 whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: PC-3 whole cell lysates,
Lane 5: HEPA1-6 whole cell lysates.
Use rabbit anti- GGCX 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GGCX at approximately 88KD. The expected band size for GGCX is at 88KD.|Figure 2. IHC analysis using anti- GGCX Antibody (A02643-1). detected in paraffin-embedded section of huamn hepatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. ICC analysis using anti- GGCX Antibody (A02643-1). was detected in immersion fixed CACO-2 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 4. Flow cytometry analysis of U251 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: MCF-7 whole cell lysates,
Lane 2: HEK293 whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: PC-3 whole cell lysates,
Lane 5: HEPA1-6 whole cell lysates.
Use rabbit anti- GGCX 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GGCX at approximately 88KD. The expected band size for GGCX is at 88KD.|Figure 2. IHC analysis using anti- GGCX Antibody (A02643-1). detected in paraffin-embedded section of huamn hepatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. ICC analysis using anti- GGCX Antibody (A02643-1). was detected in immersion fixed CACO-2 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 4. Flow cytometry analysis of U251 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-GGCX Antibody(A02643-1-100ul)
￥1960
