- ￥1960
- BOSTER
- A08969-1-100ul
- 中国
- 2025年07月16日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A08969-1 |
|---|---|
| 产品名称 | Anti-PITRM1 Antibody |
| 基因名 | PITRM1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 117KD |
| 免疫原 | E.coli-derived human PITRM1 recombinant protein (Position: A33-K1037). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Pitrilysin metallopeptidase 1 also known as presequence protease, mitochondrial (PreP) and metalloprotease 1 (MTP-1) is an enzyme that in humans is encoded by the PITRM1 gene. The protein encoded by this gene is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease. |
| 研究类别 | 1. Falkevall, A., Alikhani, N., Bhushan, S., Pavlov, P. F., Busch, K., Johnson, K. A., Eneqvist, T., Tjernberg, L., Ankarcrona, M., Glaser, E. Degradation of the amyloid beta-protein by the novel mitochondrial peptidasome, PreP. J. Biol. Chem. 281: 29096-29104, 2006.2. Mzhavia, N., Berman, Y. L., Qian, Y., Yan, L., Devi, L. A. Cloning, expression, and characterization of human metalloprotease 1: a novel member of the pitrilysin family of metalloendoproteases. DNA Cell Biol. 18: 369-380, 1999. 3. Teixeira, P. F., Pinho, C. M., Branca, R. M., Lehtio, J., Levine, R. L., Glaser, E. In vitro oxidative inactivation of human presequence protease (hPreP). Free Radic. Biol. Med. 53: 2188-2195, 2012. |
| Uniprot ID | PITRM1: Q5JRX3 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- PITRM1 Antibody (A08969-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: U2OS whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: U-87MG whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat kidneyl tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse kidney tissue lysates.
Use rabbit anti- PITRM1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PITRM1 at approximately 117KD. The expected band size for PITRM1 is at 117KD.|Figure 2. ICC analysis using anti- PITRM1 Antibody (A08969-1). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1142) and counterstained with DAPI (blue).|Figure 3. Flow cytometry analysis of U937 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: A549 whole cell lysates,
Lane 2: U2OS whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: U-87MG whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat kidneyl tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse kidney tissue lysates.
Use rabbit anti- PITRM1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PITRM1 at approximately 117KD. The expected band size for PITRM1 is at 117KD.|Figure 2. ICC analysis using anti- PITRM1 Antibody (A08969-1). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1142) and counterstained with DAPI (blue).|Figure 3. Flow cytometry analysis of U937 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-PITRM1 Antibody(A08969-1-100ul)
￥1960
