- ￥1180
- BOSTER
- M02172-1-50ul
- 中国
- 2025年07月11日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M02172-1 |
|---|---|
| 产品名称 | Anti-MFN1 Antibody(monoclonal, 3H3) |
| 基因名 | MFN1 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:3H3) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 84KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1 (23-49aa DQLLEFVTEGSHFVEATYKNPELDRIA),different from the related mouse and rat sequences by one amino acid. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80 90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed,and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion,and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers,either with itself or MFN 2,and to undergo ubiquitination by MARCH5. MFN 1 has two key domains. One is a coiled°Coil region that mediates MFN 1: MFN 1/2 binding,and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering. |
| Uniprot ID | MFN1: Q8IWA4 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- MFN1 antibody (M02172-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Use mouse anti- MFN1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001).A specific band was detected for MFN1 at approximately 84KD. The expected band size for MFN1 is at 84KD.|Figure 2. ICC analysis of anti- MFN1 antibody (M02172-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1032)and counterstained with DAPI (blue).[/list_product_images]
Lane 1: human Hela tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Use mouse anti- MFN1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001).A specific band was detected for MFN1 at approximately 84KD. The expected band size for MFN1 is at 84KD.|Figure 2. ICC analysis of anti- MFN1 antibody (M02172-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1032)and counterstained with DAPI (blue).[/list_product_images]
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Anti-Mitofusin 1 Antibody (monoclonal,3H3）(M02172-1-50ul)
￥1180
