A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin IV(7-21aa TVKAASGFNAMEDAQ), different from the related mouse and rat sequences by one amino acid.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Annexin IV, also known as placental anticoagulant protein II, or zymogen granule membrane-associated protein, 36-KD(ZAP36), is one of the family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV has 45 to 59% identity with other members of the annexin family. Annexin IV gene spans 18 to 56 kb. And it is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. Rat annexin IV is ubiquitously expressed in epithelial tissues. Zap36 associated with zymogen granule membranes in dog and rat pancreas. Human annexin IV gene is mapped to chromosome 2p13.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- ANXA4 antibody (PA1007-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human U251 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human PC-3 whole cell lysates,
Lane 8: rat lung tissue lysates,
Lane 9: mouse lung tissue lysates.
Use rabbit anti- ANXA4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ANXA4 at approximately 36KD. The expected band size for ANXA4 is at 36KD.|Anti-Annexin IV antibody, PA1007-1, IHC(P) IHC(P): Human Mammary Cancer Tissue|Anti-Annexin IV antibody, PA1007-1, IHC(P) IHC(P): Rat Pancreas Tissue[/list_product_images]