E.coli-derived human MGP recombinant protein (Position: Y20-F96). Human MGP shares 84.4% and 80.5% amino acid (aa) sequence identity with mouse and rat MGP, respectively.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Matrix Gla protein (MGP) is an 84-residue vitamin K-dependent protein initially isolated from bovine bone. In addition, MGP is a 10-kD protein produced and secreted by vascular smooth muscle cells and chondrocytes and significantly accumulated in bone, cartilage, and dentin. It is also expressed at high levels in heart, kidney, and lung and is upregulated by vitamin D in bone cells. MGP has a high affinity binding to calcium ions, similar to other Gla-containing proteins. The protein acts as an inhibitor of vascular mineralization and plays a role in bone organization. And this gene is mapped to 12p12.3.
研究类别
1. Hur, D. J., Raymond, G. V., Kahler, S. G., Riegert-Johnson, D. L., Cohen, B. A., Boyadjiev, S. A. A novel MGP mutation in a consanguineous family: review of the clinical and molecular characteristics of Keutel syndrome. Am. J. Med. Genet. 135A: 36-40, 2005.2. Luo G, Ducy P, McKee MD, Pinero GJ, Loyer E, Behringer RR, Karsenty G (March 1997). "Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein". Nature 386 (6620): 78–81.3. Yao Y, Jumabay M, Ly A, Radparvar M, Cubberly MR, Bostr m KI (2013). "A role for the endothelium in vascular calcification". Circ. Res. 113 (5): 495–504.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Western blot analysis of MGP expression in JURKAT whole cell lysates (lane 1). MGP at 12KD was detected using rabbit anti- MGP Antigen Affinity purified polyclonal antibody (Catalog # PB9954) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|MGP was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- MGP Antigen Affinity purified polyclonal antibody (Catalog # PB9954) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).[/list_product_images]