500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin-dependent kinases. P19(INK4d) also kowns as cyclin-dependent kinase inhibitor 2D, is one of the novel members of the mouse INK4 gene family. Okuda et al.(1995) described the cloning of the human INK4d gene(CDKN2D). The predicted 166-amino acid protein is 86% identical to the mouse protein and about 45% identical to other human INK4 family members.
研究类别
1. Hirai, H.; Roussel, M. F.; Kato, J.-Y.; Ashmun, R. A.; Sherr, C. J. : Novel INK4 proteins, p19 and p18, are specific inhibitors of cyclin D-dependent kinases CDK4 and CDK6. Molec. Cell. Biol. 15: 2672-2681, 1995. 2. tsuzaki, Y.; Miyazawa, K.; Yokota, T.; Hitomi, T.; Yamagishi, H.; Sakai, T. : Molecular cloning and characterization of the human p19(INK4d) gene promoter. FEBS Lett. 517: 272-276, 2002.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- CDKN2D antibody (MA1075). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MOLT-4 whole cell lysates,
Lane 2: HEL whole cell lysates,
Lane 3: Jurkat whole cell lysates.
Use mouse anti- CDKN2D 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CDKN2D at approximately 18KD. The expected band size for CDKN2D is at 18KD.[/list_product_images]