- ￥1960
- BOSTER
- BA3201-2-100ul
- 中国
- 2025年07月08日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BA3201-2 |
|---|---|
| 产品名称 | Anti-LAMA2 Antibody |
| 基因名 | LAMA2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 344KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human Laminin 2 alpha(152-170aa WILERSLDDVEYKPWQYHA), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Laminin, alpha-2, also known as LAMA2, is a protein that in humans is encoded by the LAMA2 gene. This gene is mapped to 6q22.33. Laminin, an extracellular protein, is a major component of the basement membrane. It is though to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2(merosin) and laminin 4(s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. |
| 研究类别 | 1. Coral-Vazquez, R. M., Rosas-Vargas, H., Meza-Espinosa, P., Mendoza, I., Huicochea, J. C., Ramon, G., Salamanca, F. Severe congenital muscular dystrophy in a Mexican family with a new nonsense mutation (R2578X) in the laminin alpha-2 gene. J. Hum. Genet. 48: 91-95, 2003.2. Millay, D. P., Sargent, M. A., Osinska, H., Baines, C. P., Barton, E. R., Vuagniaux, G., Sweeney, H. L., Robbins, J., Molkentin, J. D. Genetic and pharmacologic inhibition of mitochondrial-dependent necrosis attenuates muscular dystrophy. Nature Med. 14: 442-447, 2008. |
| Uniprot ID | LAMA2: P24043 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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文献和实验Purification Of Anti Peptide Antibody
a parastaltic pump and recirculate the serum ON or just batch bind the serum ON. Wash with 5 col vols TBS. Wash with 10 col vols 0.5 M NaCl, 20mM TrisCl pH 7.4, 0.2 Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine
Purification Of Anti Peptide Antibody
Purification Of Anti Peptide Antibody Claire Walczak January 1996 Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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