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- BOSTER
- A15490-1-50ul
- 中国
- 2025年07月11日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A15490-1 |
|---|---|
| 产品名称 | Anti-HOXC12 Antibody |
| 基因名 | HOXC12 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 35KD |
| 免疫原 | E.coli-derived human HOXC12 recombinant protein (Position: M1-N195). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Homeobox C12 is a protein that in humans is encoded by the HOXC12 gene. This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. |
| 研究类别 | 1. Kang, X. , Qi, Y. , Yong, Z. , Qi, W. , & Yeh, E. . (2010). Sumo-specific protease 2 is essential for suppression of polycomb group protein-mediated gene silencing during embryonic development. Molecular Cell, 38(2), 191-201.2. Grier, D. G. , Thompson, A. , Lappin, T. , & Halliday, H. L. . (2009). Quantification of hox and surfactant protein-b transcription during murine lung development. Neonatology, 96(1), 50-60.3. Katayama, K. , Furuno, A. , Akiyama, K. , Tsuji, T. , & Kunieda, T. . (2007). Characterization of chromosomal inversion of the mouse hairy ears (eh) mutation associated with cleft palate. Mammalian Genome, 18(4), 246-254. |
| Uniprot ID | HOXC12: P31275 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- HOXC12 antibody (A15490-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hacat whole cell lysates.
Use rabbit anti- HOXC12 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HOXC12 at approximately 35KD. The expected band size for HOXC12 is at 30KD.|Figure 2. IHC analysis using anti- HOXC12 antibody (A15490-1). detected in paraffin-embedded section of mouse skin tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.[/list_product_images]
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hacat whole cell lysates.
Use rabbit anti- HOXC12 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HOXC12 at approximately 35KD. The expected band size for HOXC12 is at 30KD.|Figure 2. IHC analysis using anti- HOXC12 antibody (A15490-1). detected in paraffin-embedded section of mouse skin tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.[/list_product_images]
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Anti-HOXC12 Antibody(A15490-1-50ul)
￥1180
