- ￥1180
- BOSTER
- A03298-1-50ul
- 中国
- 2025年07月06日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A03298-1 |
|---|---|
| 产品名称 | Anti-GSTM3 Antibody |
| 基因名 | GSTM3 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 26KD |
| 免疫原 | E. coli-derived human GSTM3 recombinant protein (Position: E93-Q206). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Glutathione S-transferase M3 (brain), also known as GSTM3, is an enzyme which in humans is encoded by theGSTM3 gene. Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. |
| 研究类别 | 1. "Entrez Gene: GSTM3 glutathione S-transferase M3 (brain)".2. Campbell E, Takahashi Y, Abramovitz M, Peretz M, Listowsky I (June 1990). "A distinct human testis and brain mu-class glutathione S-transferase. Molecular cloning and characterization of a form present even in individuals lacking hepatic type mu isoenzymes". J. Biol. Chem. 265 (16): 9188–93. |
| Uniprot ID | GSTM3: P21266 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of GSTM3 using anti-GSTM3 antibody (A03298-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate,Lane 2: rat brain tissue lysate,Lane 3: mouse testis tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: human Hela cell lysate,Lane 6: human MCF-7 cell lysate. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for GSTM3 at approximately 26KD. The expected band size for GSTM3 is at 26KD.Figure 2. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).GSTM3 was detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).GSTM3 was detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).GSTM3 was detected in paraffin-embedded section of human testis tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).GSTM3 was detected in paraffin-embedded section of mouse brain tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).GSTM3 was detected in paraffin-embedded section of mouse testis tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).GSTM3 was detected in paraffin-embedded section of rat brain tissue . anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).GSTM3 was detected in paraffin-embedded section of rat testis tissue . anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]
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Anti-GSTM3 Antibody(A03298-1-50ul)
￥1180
