- ￥1180
- BOSTER
- A00049-5-50ul
- 中国
- 2025年07月06日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00049-5 |
|---|---|
| 产品名称 | Anti-ABCB1 Antibody |
| 基因名 | ABCB1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 130-180KD |
| 免疫原 | E.coli-derived human P Glycoprotein/ABCB1 recombinant protein (Position: M1-E690). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood–brain barrier. |
| 研究类别 | 1. Aller SG, Yu J, Ward A, Weng Y, Chittaboina S, Zhuo R, Harrell PM, Trinh YT, Zhang Q, Urbatsch IL, Chang G (March 2009).2. Ueda K, Clark DP, Chen CJ, Roninson IB,Gottesman MM, Pastan I (January 1987). "The human multidrug resistance (mdr1) gene. cDNA cloning and transcription initiation". J. Biol. Chem.262 (2): 505–8. |
| Uniprot ID | ABCB1: P08183 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- ABCB1 antibody (A00049-5). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: DLD1 whole cell lysates,
Lane 2: HUH-7 whole cell lysates.
Use rabbit anti- ABCB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ABCB1 at approximately 160KD. The expected band size for ABCB1 is at 140KD.|Figure 2. IHC analysis using anti- ABCB1 antibody (A00049-5). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ABCB1 antibody (A00049-5). detected in paraffin-embedded section of human thyroid cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of CACO2 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).DAPI (blue).[/list_product_images]
Lane 1: DLD1 whole cell lysates,
Lane 2: HUH-7 whole cell lysates.
Use rabbit anti- ABCB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ABCB1 at approximately 160KD. The expected band size for ABCB1 is at 140KD.|Figure 2. IHC analysis using anti- ABCB1 antibody (A00049-5). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ABCB1 antibody (A00049-5). detected in paraffin-embedded section of human thyroid cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of CACO2 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).DAPI (blue).[/list_product_images]
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Anti-P Glycoprotein/ABCB1 Antibody(A00049-5-50ul)
￥1180
