Anti-RAB27A Antibody (monoclon

[list_product_images]Figure 1. Western blot analysis of RAB27A using anti-RAB27A antibody (M01608).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane 1: human K562 whole cell lysates

Lane 2: human HepG2 whole cell lysates

Lane 3: human THP-1 whole cell lysates

Lane 4: human HT1080 whole cell lysates

Lane 5: human SW620 whole cell lysates

Lane 6: human PANC-1 whole cell lysates

Lane 7: rat RH35 whole cell lysates

Lane 8: mouse NIH/3T3 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAB27A antigen affinity purified monoclonal antibody (Catalog # M01608) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RAB27A at approximately 27KD. The expected band size for RAB27A is at 27KD.|Figure 2. IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
RAB27A was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
RAB27A was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
RAB27A was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
RAB27A was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
RAB27A was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.|Figure 7. Flow Cytometry analysis of K562 cells using anti-RAB27A antibody (M01608).
Overlay histogram showing K562 cells stained with M01608 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB27A Antibody (M01608,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 8. Flow Cytometry analysis of U87 cells using anti-RAB27A antibody (M01608).
Overlay histogram showing U87 cells stained with M01608 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB27A Antibody (M01608,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 9.ICC analysis using anti-RAB27A antibody (M01608) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).[/list_product_images]