- ￥1180
- BOSTER
- M01561-2-50ul
- 中国
- 2025年07月12日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M01561-2 |
|---|---|
| 产品名称 | Anti-ITGAV Antibody |
| 基因名 | ITGAV |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:8B10H2） |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 130-140KD |
| 免疫原 | E.coli-derived human Integrin alpha V/ITGAV recombinant protein (Position: H732-D970). |
| 内容 | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| 纯化方式 | protein G/A purified |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Integrin alpha-V is a protein that in humans is encoded by the ITGAV gene. It is a member of the beta 3 integrin subfamily(cytoadhesins). The human locus for the av gene(VNRA) was previously mapped to the long arm of chromosome 2. Sims et al.(2000) localized the VNRA gene to 2q31. The gene contains 30 exons and spans over 93 kb of genomic DNA. It functions as a receptor for a group of proteins that includes vitronectin, fibrinogen, thrombospondin, and von Willebrand factor. |
| 研究类别 | 1. Sosnoski DM, Emanuel BS, Hawkins AL, van Tuinen P, Ledbetter DH, Nussbaum RL, Kaos FT, Schwartz E, Phillips D, Bennett JS, et al. (Aug 1988). "Chromosomal localization of the genes for the vitronectin and fibronectin receptors alpha subunits and for platelet glycoproteins IIb and IIIa". J Clin Invest 81 (6): 1993–8. 2. Fernandez-Ruiz, E., de Villena, F. P.-M., Rodriguez de Cordoba, S., Sanchez-Madrid, F.Regional localization of the human vitronectin receptor alpha-subunit gene (VNRA) to chromosome 2q31-q32.Cytogenet. Cell Genet.62: 26-28, 1993. |
| Uniprot ID | ITGAV: P06756 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- V/ITGAV antibody (M01561-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: A431 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: MCF-7 whole cell lysates.
Use mouse anti- V/ITGAV 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for V/ITGAV at approximately 130KD. The expected band size for V/ITGAV is at 116KD.|Figure 2. ICC analysis using anti- V/ITGAV antibody (M01561-2). was detected in immersion fixed A549 cell. Cells were stained using the cy3-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1031).and counterstained with DAPI (blue).|Figure 3. IHC analysis using anti- alpha V/ITGAV antibody (M01561-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- alpha V/ITGAV antibody (M01561-2). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]
Lane 1: A549 whole cell lysates,
Lane 2: A431 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: MCF-7 whole cell lysates.
Use mouse anti- V/ITGAV 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for V/ITGAV at approximately 130KD. The expected band size for V/ITGAV is at 116KD.|Figure 2. ICC analysis using anti- V/ITGAV antibody (M01561-2). was detected in immersion fixed A549 cell. Cells were stained using the cy3-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1031).and counterstained with DAPI (blue).|Figure 3. IHC analysis using anti- alpha V/ITGAV antibody (M01561-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- alpha V/ITGAV antibody (M01561-2). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]
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Anti-Integrin alpha V/ITGAV Antibody (monoclonal, 8B10H2)(M01561-2-50ul)
￥1180
