- ￥1960
- BOSTER
- A10474-2-100ul
- 中国
- 2025年07月16日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A10474-2 |
|---|---|
| 产品名称 | Anti-PPP1R11 Antibody |
| 基因名 | PPP1R11 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 25KD |
| 免疫原 | E.coli-derived human PPP1R11 recombinant protein (Position: M1-H126). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Protein phosphatase 1 regulatory subunit 11 is an enzyme that in humans is encoded by the PPP1R11 gene. This gene encodes a specific inhibitor of protein phosphatase-1 (PP1) with a differential sensitivity toward the metal-independent and metal-dependent forms of PP1. The gene is located within the major histocompatibility complex class I region on chromosome 6. |
| 研究类别 | 1. Amadou, C., Ribouchon, M. T., Mattei, M. G., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., Avoustin, P., Pontarotti, P. Localization of new genes and markers to the distal part of the human major histocompatibility complex (MHC) region and comparison with the mouse: new insights into the evolution of mammalian genomes. Genomics 26: 9-20, 1995.2. el Kahloun, A., Chauvel, B., Mauvieux, V., Dorval, I., Jouanolle, A.-M., Gicquel, I., Le Gall, J.-Y., David, V. Localization of seven new genes around the HLA-A locus. Hum. Molec. Genet. 2: 55-60, 1993. 3. Giffon, T., Lepourcelet, M., Pichon, L., Jezequel, P., Bouric, P., Carn, G., Pontarotti, P., Le Gall, J.-Y., David, V. Cloning of a human homologue of the mouse Tctex-5 gene within the MHC class I region. Immunogenetics 44: 331-339, 1996. |
| Uniprot ID | PPP1R11: O60927 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-PPP1R11 antibody (A10474-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPP1R11 antigen affinity purified polyclonal antibody (A10474-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PPP1R11 at approximately 25 kDa. The expected band size for PPP1R11 is at 14,18,25-30 kDa.|Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-PPP1R11 antibody (A10474-2).
Overlay histogram showing MCF-7 cells stained with A10474-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R11 Antibody (A10474-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPP1R11 antigen affinity purified polyclonal antibody (A10474-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PPP1R11 at approximately 25 kDa. The expected band size for PPP1R11 is at 14,18,25-30 kDa.|Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-PPP1R11 antibody (A10474-2).
Overlay histogram showing MCF-7 cells stained with A10474-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R11 Antibody (A10474-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-PPP1R11 Antibody(A10474-2-100ul)
￥1960
