- ￥1180
- BOSTER
- A04265-2-50ul
- 中国
- 2025年07月06日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A04265-2 |
|---|---|
| 产品名称 | Anti-EHD2 Antibody |
| 基因名 | EHD2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 61KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human EHD2 (KKQLILKLPVIFAKIQLEHH). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | EH-domain containing 2, also known as EHD2, is a human gene[5] belonging to the EHD protein family. This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. |
| 研究类别 | 1. Daumke, O., Lundmark, R., Vallis, Y., Martens, S., Butler, P. J. G., McMahon, H. T. Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling. Nature 449: 923-927, 2007. 2. Guilherme, A., Soriano, N. A., Bose, S., Holik, J., Bose, A., Pomerleau, D. P., Furcinitti, P., Leszyk, J., Corvera, S., Czech, M. P. EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton. J. Biol. Chem. 279: 10593-10605, 2004.3. Pohl, U., Smith, J. S., Tachibana, I., Ueki, K., Lee, H. K., Ramaswamy, S., Wu, Q., Mohrenweiser, H. W., Jenkins, R. B., Louis, D. N. EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins. Genomics 63: 255-262, 2000. |
| Uniprot ID | EHD2: Q9NZN4 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- EHD2 Antibody (A04265-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U-87MG whole cell lysates.
Use rabbit anti- EHD2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for EHD2 at approximately 61KD. The expected band size for EHD2 is at 51KD.|Figure 2. ICC analysis using anti- EHD2 Antibody (A04265-2). was detected in immersion fixed A549 cell line. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1142) and counterstained with DAPI (blue).[/list_product_images]
Lane 1: U-87MG whole cell lysates.
Use rabbit anti- EHD2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for EHD2 at approximately 61KD. The expected band size for EHD2 is at 51KD.|Figure 2. ICC analysis using anti- EHD2 Antibody (A04265-2). was detected in immersion fixed A549 cell line. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1142) and counterstained with DAPI (blue).[/list_product_images]
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Anti-EHD2 Antibody(A04265-2-50ul)
￥1180
