- ￥1960
- BOSTER
- A01626-4-100ul
- 中国
- 2025年07月06日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A01626-4 |
|---|---|
| 产品名称 | Anti-TPH1 Antibody |
| 基因名 | TPH1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 60KD |
| 免疫原 | E.coli-derived human Tryptophan Hydroxylase/TPH1 recombinant protein (Position: K383-I444). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Tryptophan hydroxylase 1 (TPH1) is an isoenzyme of tryptophan hydroxylase which in humans is encoded by the TPH1 gene. This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others. |
| 研究类别 | 1. "Entrez Gene: TPH1 tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)".2. New AS, Gelernter J, Yovell Y, Trestman RL, Nielsen DA, Silverman J, Mitropoulou V, Siever LJ (1998). "Tryptophan hydroxylase genotype is associated with impulsive-aggression measures: a preliminary study". Am. J. Med. Genet. 81 (1): 13–7. |
| Uniprot ID | TPH1: P17752 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- TPH1 Antibody (A01626-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: HL-60 whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: Caco-2 whole cell lysates.
Use rabbit anti- TPH1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TPH1 at approximately 60KD. The expected band size for TPH1 is at 51KD.|Figure 2. IHC analysis using anti- TPH1 Antibody (A01626-4). detected in paraffin-embedded section of human bladder epithelial carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- TPH1 Antibody (A01626-4). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- TPH1 Antibody (A01626-4). detected in paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. ICC analysis using anti- TPH1 Antibody (A01626-4). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 6. Flow cytometry analysis of HEL cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: 293T whole cell lysates,
Lane 2: HL-60 whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: Caco-2 whole cell lysates.
Use rabbit anti- TPH1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TPH1 at approximately 60KD. The expected band size for TPH1 is at 51KD.|Figure 2. IHC analysis using anti- TPH1 Antibody (A01626-4). detected in paraffin-embedded section of human bladder epithelial carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- TPH1 Antibody (A01626-4). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- TPH1 Antibody (A01626-4). detected in paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. ICC analysis using anti- TPH1 Antibody (A01626-4). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 6. Flow cytometry analysis of HEL cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-TPH1 Antibody(A01626-4-100ul)
￥1960
