- ￥1180
- BOSTER
- PA1007-1-50ul
- 中国
- 2025年07月07日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PA1007-1 |
|---|---|
| 产品名称 | Anti-ANXA4 Antibody |
| 基因名 | ANXA4 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 36KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin IV(7-21aa TVKAASGFNAMEDAQ), different from the related mouse and rat sequences by one amino acid. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Annexin IV, also known as placental anticoagulant protein II, or zymogen granule membrane-associated protein, 36-KD(ZAP36), is one of the family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV has 45 to 59% identity with other members of the annexin family. Annexin IV gene spans 18 to 56 kb. And it is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. Rat annexin IV is ubiquitously expressed in epithelial tissues. Zap36 associated with zymogen granule membranes in dog and rat pancreas. Human annexin IV gene is mapped to chromosome 2p13. |
| Uniprot ID | ANXA4: P09525 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:20-100 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- ANXA4 antibody (PA1007-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human U251 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human PC-3 whole cell lysates,
Lane 8: rat lung tissue lysates,
Lane 9: mouse lung tissue lysates.
Use rabbit anti- ANXA4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ANXA4 at approximately 36KD. The expected band size for ANXA4 is at 36KD.|Anti-Annexin IV antibody, PA1007-1, IHC(P) IHC(P): Human Mammary Cancer Tissue|Anti-Annexin IV antibody, PA1007-1, IHC(P) IHC(P): Rat Pancreas Tissue[/list_product_images]
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human U251 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human PC-3 whole cell lysates,
Lane 8: rat lung tissue lysates,
Lane 9: mouse lung tissue lysates.
Use rabbit anti- ANXA4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ANXA4 at approximately 36KD. The expected band size for ANXA4 is at 36KD.|Anti-Annexin IV antibody, PA1007-1, IHC(P) IHC(P): Human Mammary Cancer Tissue|Anti-Annexin IV antibody, PA1007-1, IHC(P) IHC(P): Rat Pancreas Tissue[/list_product_images]
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Anti-Annexin IV Antibody(PA1007-1-50ul)
￥1180
