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- BOSTER
- A05806-50ul
- 中国
- 2025年07月12日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A05806 |
|---|---|
| 产品名称 | Anti-HNRNPF Antibody |
| 基因名 | HNRNPF |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 45KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F (23-53aa SVEDVQNFLSDCTIHDGAAGVHFIYTREGRQ), different from the related mouse and rat sequences by two amino acids. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| 研究类别 | 1. "Entrez Gene: HNRPF heterogeneous nuclear ribonucleoprotein F".2. Honore B, Rasmussen HH, Vorum H, Dejgaard K, Liu X, Gromov P, Madsen P, Gesser B, Tommerup N, Celis JE (Jan 1996). "Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes". J Biol Chem. 270 (48): 28780–9.3. Matunis MJ, Xing J, Dreyfuss G (1994). "The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.". Nucleic Acids Res. 22 (6): 1059–67. |
| Uniprot ID | HNRNPF: P52597 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in frozen section: | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of HnRNPF using anti-HnRNPF antibody (A05806).
lane 1: rat liver tissue lysate,
lane 2: mouse spleen tissue lysate,
lane 3: HELA whole cell lysates.
anti-HnRNPF antigen affinity purified polyclonal antibody (Catalog # A05806)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for HnRNPF at approximately 45KD. The expected band size for HnRNPF is at 45KD.|Figure 2. IHC analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in paraffin-embedded section of human intetsinal cancer tissues. anti-HnRNPF Antibody (A05806) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in paraffin-embedded section of human mammary cancer tissues. anti-HnRNPF Antibody (A05806) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IF analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in immunocytochemical section of SKOV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti-HnRNPF Antibody (A05806) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 5. IHC analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in paraffin-embedded section of mouse intestine tissues. anti-HnRNPF Antibody (A05806) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in paraffin-embedded section of rat intestine tissues. anti-HnRNPF Antibody (A05806) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 7. Flow Cytometry analysis of U87 cells using anti-HnRNPF antibody (A05806).
Overlay histogram showing U87 cells stained with A05806 (Blue line).anti-HnRNPF Antibody (A05806, 1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
lane 1: rat liver tissue lysate,
lane 2: mouse spleen tissue lysate,
lane 3: HELA whole cell lysates.
anti-HnRNPF antigen affinity purified polyclonal antibody (Catalog # A05806)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for HnRNPF at approximately 45KD. The expected band size for HnRNPF is at 45KD.|Figure 2. IHC analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in paraffin-embedded section of human intetsinal cancer tissues. anti-HnRNPF Antibody (A05806) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in paraffin-embedded section of human mammary cancer tissues. anti-HnRNPF Antibody (A05806) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IF analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in immunocytochemical section of SKOV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti-HnRNPF Antibody (A05806) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 5. IHC analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in paraffin-embedded section of mouse intestine tissues. anti-HnRNPF Antibody (A05806) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis of HnRNPF using anti-HnRNPF antibody (A05806).
HnRNPF was detected in paraffin-embedded section of rat intestine tissues. anti-HnRNPF Antibody (A05806) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 7. Flow Cytometry analysis of U87 cells using anti-HnRNPF antibody (A05806).
Overlay histogram showing U87 cells stained with A05806 (Blue line).anti-HnRNPF Antibody (A05806, 1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-HnRNP F/HNRNPF Antibody(A05806-50ul)
￥1180
