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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
HNRNPF
- 浓度:
1 mg/ml
- 应用范围:
WB 1:2000, IHC 1:50, IF 1:50~100, FLOW 1:100
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 标记物:
Unconjugated
- 克隆性:
Monoclonal Antibody
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态:
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
- 亚型:
IgG2a
- 免疫原:
Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
- 规格:
100 ul/30 ul
| 规格: | 100 ul | 产品价格: | ¥2025.0 |
|---|---|---|---|
| 规格: | 30 ul | 产品价格: | ¥690.0 |
产品概述:HNRNPF (hnRNP F) mouse monoclonal antibody, clone LBI5F5
总结:This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
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