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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C, sealed storage, away from moisture and light
- 库存:
货期:询盘
- 供应商:
MedChemExpress LLC
- 规格:
100 μL///200 μL
MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
Cy5-UTP sodium
MCE 国际站:Cy5-UTP sodium
产品活性:Cy5-UTP sodium 是一种 Cyanine5 标记的 UTP 类似物,在体外转录反应中可以产生荧光探针,激发/发射波长为 650/665 nm。Cy5-UTP sodium 可应用于 FISH,多色荧光分析,特别是与 Cy5-UTP结合的双色表达阵列。
研究领域:Others
作用靶点:Others
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文献和实验polymerase as appropriate 2. Mix then spin down briefly. 3. Incubate at 37'C for 2-3 hours. 4. Add 2 ul DNase I, RNase free to remove DNA template. 5. Incubate at 37'C for 15 minutes. 10X NTP Labeling Mix: 10mM ATP, CTP, GTP, 6.5mM UTP, 3.5mM DIG in Tris
for 15 minutes. 10X NTP Labeling Mix: 10mM ATP, CTP, GTP, 6.5mM UTP, 3.5mM DIG in Tris-HCl, pH 7.5. 10X Transcription Buffer: 400mM Tris-HCl, pH 8.0; 60mM MgCl2, 100mM dithioerythritol, 20mM spermidine, 100mM NaCl, 1U/ml RNase Inhibitor
PCR clean up using 3M sodium acetate and chilled absolute ethanol
For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube. 2 ul of 3M sodium acetate (NaOAc) pH 4.5 40 ul chilled absolute ethanol. Transfer the PCR product (20ul) into the tube containing
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