种属:Human
分子:CXCL10
应用:ELISA
保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
包被抗体:1 mg/mL of mouse anti-Human IP-10/CXCL10 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10768-MM07)
检测抗体:0.2 mg/mL of mouse anti-Human IP-10/CXCL10 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in dilution buffer before use. (Catalog: # 10768-MM10)
标准品:Each vial contains 22 ng of recombinant Human IP-10/CXCL10. Reconstitute with 1 mL Standard dissolution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 350 pg/mL is recommended.
灵敏度:The minimum detectable dose of Human IP-10/CXCL10 was determined to be approximately 5.47 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
检测原理:The Human IP-10/CXCL10 ELISA Pair Set is for the quantitative determination of Human IP-10/CXCL10.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human IP-10/CXCL10 coated on a 96-well plate. Standards and samples are added to the wells, and any Human IP-10/CXCL10 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human IP-10/CXCL10 monoclonal antibody is then added, producing an antibody-antigen-antibody ""sandwich"". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human IP-10/CXCL10 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
检测限:5.47 pg/mL