重组人 CLDN6 蛋白-(Nanodisc) (His & AVI 标签), 生物素标记

规格/价格
20 µg：19470
100 µg：68220

CLDN6 Protein, Human, Recombinant (His & AVI Tag), Biotinylated, Nanodisc: 产品信息

• 纯度：≥ 75 % as determined by SDS-PAGE.
• 内毒素：< 1.0 EU per μg protein as determined by the LAL method.
• 生物活性：Immobilized Recombinant Human CLDN6 -Nanodisc Protein(His & AVI Tag), Biotinylated at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL) can bind Anti-Human Claudin6 Antibody, Human IgG1, the EC50 is 2-6 ng/mL.
• 蛋白构建：A DNA sequence encoding the human CLDN6 (P56747) (1-184) was expressed, with a polyhistidine tag and AVI tag at the C-terminus. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
• 表达宿主：HEK293 Cells
• 种属：Human
• 预测 N 端：Met 1
• 分子量：The recombinant human Claudin-6 consists of 209 amino acids and has a predicted molecular mass of 22.7 kDa. The apparent molecular mass of it is approximately 23 KDa in SDS-PAGE under reducing conditions.
• 缓冲液

1. Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH 7.5 with glycerol as protectant.
2. Please contact us for any concerns or special requirements.
3. Please refer to the specific buffer information in the hard copy of CoA.

• 运输方式：It is supplied and shipped as liquid with dry ice.
• 稳定性 & 储存条件

1. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
2. Samples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• 复溶：A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

CLDN6 背景信息
Claudin-6 (CLDN6) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and intracellular N and C tails. CLDN6 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It regulates the paracellular flux of cations in the kidney through forming heterotypic strands with other claudins and has a role in maintaining the lung epithelial barrier function. The CLDN6 gene is adjacent to another family member, CLDN9, on chromosome 16. Certain CLDN6-positive cancers, encompassing ovarian, endometrial, and testicular malignancies, have been shown to have their proliferation and invasiveness suppressed upon CLDN6 silencing.

全称
claudin 6