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- 2025年07月16日
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文献和实验Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis
The plasma membrane (PM) exists as the interface between the cytosol and the environment in all living cells and is one of the most complex and differentiated membrane. The identification and characterization of membrane proteins
Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay
Preparation of plasma membrane (PM) sheets from intact cells is a simple method for obtaining an immobilized cytoplasmic PM surface that can be probed with antibodies or chemical probes. This method was first reported by Robinson et al
Expression of Recombinant Membrane-Type MMPs
” (1 ) there has been much interest in the production of recombinant protein to facilitate both structural and functional analyses. The proteolytic activity of the membrane-type-1 MMP (MT1-MMP) has been studied using recombinant material produced from expression in E. coli (2 ,3 ). These data
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