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- 2025年07月15日
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
kidney D-amino-acid oxidase (Sigma, St. Louis, MO or Boehringer Mannheim, Germany) was used as a control. Organs, tissues, or tissue culture cells were homogenized in distilled water and centrifuged at 550 x g for 5 min. The supernatant solutions
Methods for the Detection of D-Amino-Acid Oxidase
phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution ( 8 ), and 0.5% SDS] containing 20 μg/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
of overexpression strategy. Curr. Protoc. Protein Sci. 72:27.4.1?27.4.14. © 2013 by John Wiley & Sons, Inc. Keywords: protein array; post?translation; phosphorylation; kinase
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