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文献和实验Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) could be detected in the bound fraction after precipitation with conventional mono- and polyclonal antibodies (Figure 1) (Rothbauer et al., 2008). The lack of unspecific binding or antibody fragments is one major advantage of the GFP-Trap, because unspecific protein
Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
7.5), 1 mM EDTA, 10 mM MgCl 2 , 10 µM GTP, 10 µg/ml GDP antibody, and 2 nM fluorescent nucleotide tracer; for the protocol below, this will be prepared as a 1.1× solution (18 µl added to a final 20 µl
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
sapiens glutamate receptor, metabotropic 4 (GRM4), mRNA 1369 NM_002070 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2), mRNA 1877 NM_001493 Homo sapiens GDP dissociation inhibitor 1 (GDI1
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