- 询价
- Cytoskeleton
- GDI01-A-1 x 25 µg
- 2026年02月19日
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- 文献和实验
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- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
1 x 25 µg
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文献和实验Thaw out 1 ml aliquot of fusion protein lysate. Centrifuge to remove precipitate. Incubate 1 ml lysate with 0.4 ml 50% GST beads. Incubate at 4C with mixing for 1 hour. Centrifuge 5K 2' in 1.5 ml eppendorf tubes. Wash the beads 4X with PBST
GST Fusion Protein Preparation
CLEAVAGEAlternate Mini PrepThaw out 1 ml aliquot of fusion protein lysate.Centrifuge to remove precipitate.Incubate 1 ml lysate with 0.4 ml 50% GST beads.Incubate at 4℃ with mixing for 1 hour.Centrifuge 5K 2' in 1.5 ml eppendorf tubes.Wash the beads 4X with PBST
Thrombin Cleavage of GST-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of thrombin, temperature and length of incubation must be calibrated
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