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- PeproTech
- 130-09-5UG
- 2025年07月31日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
5ug
Human Visfatin (5ug)
Human Visfatin (1mg)
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Product Details |
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Size |
5 ug |
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Species |
Human |
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Published Species |
Human, Mouse |
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Expression system |
E. coli |
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Amino acid sequence |
MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH |
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Molecular weight |
52.6 kDa |
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Class |
Recombinant |
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Type |
Protein |
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Purity |
98% by SDS-PAGE gel and HPLC analyses. |
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Endotoxin concentration |
<1 EU/µg |
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Activity |
The ED50 was determined by the dose-dependent proliferation of the RPMI 8226 cells. The expected ED50 for this effect is 15.0-20.0 ng/ml. |
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Conjugate |
Unconjugated |
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Form |
Lyophilized |
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Purification |
purified |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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Applications |
Tested Dilution |
Publications |
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Western Blot (WB) |
Assay-dependent |
- |
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ELISA (ELISA) |
Assay-dependent |
- |
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Functional Assay (Functional) |
Assay-dependent |
- |
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In vitro Assay (IV) |
- |
5 Publications |
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Miscellaneous PubMed (Misc) |
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1 Publication |
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Product Specific Information
130-09-1MG will be provided as 2 x 500 µg (130-09-500UG).
Recombinant human Visfatin is a 52.6 kDa protein containing 466 amino acid residues (isoform 1).
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis
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文献和实验[1]Panizza E, Regalado BD, Wang F, Nakano I, Vacanti NM, Cerione RA, Antonyak MA. Proteomic analysis reveals microvesicles containing NAMPT as mediators of radioresistance in glioma. Life Sci Alliance. 2023 Apr 10;6(6):e202201680. doi: 10.26508/lsa.202201680. PMID: 37037593; PMCID: PMC10087103.
[2]Chiang YF, Huang KC, Chen HY, Huang TC, Ali M, Chang HY, Shieh TM, Shih YH, Wang KL, Huang YJ, Chung CP, Hsia SM. The Adipokine Visfatin Modulates Cancer Stem Cell Properties in Triple-Negative Breast Cancer. Biomedicines. 2023 Jan 20;11(2):297. doi: 10.3390/biomedicines11020297. PMID: 36830834; PMCID: PMC9953233.
[3]Van den Bergh R, Morin S, Sass HJ, Grzesiek S, Vekemans M, Florence E, Tran HT, Imiru RG, Heyndrickx L, Vanham G, De Baetselier P, Raes G. Monocytes contribute to differential immune pressure on R5 versus X4 HIV through the adipocytokine visfatin/NAMPT. PLoS One. 2012;7(4):e35074. doi: 10.1371/journal.pone.0035074. Epub 2012 Apr 6. PMID: 22493731; PMCID: PMC3320877.
[4]Van den Bergh R, Florence E, Vlieghe E, Boonefaes T, Grooten J, Houthuys E, Tran HT, Gali Y, De Baetselier P, Vanham G, Raes G. Transcriptome analysis of monocyte-HIV interactions. Retrovirology. 2010 Jun 14;7:53. doi: 10.1186/1742-4690-7-53. PMID: 20546557; PMCID: PMC2900222.
[5]Moschen AR, Kaser A, Enrich B, Mosheimer B, Theurl M, Niederegger H, Tilg H. Visfatin, an adipocytokine with proinflammatory and immunomodulating properties. J Immunol. 2007 Feb 1;178(3):1748-58. doi: 10.4049/jimmunol.178.3.1748. PMID: 17237424.
[6]Chiang YF, Huang KC, Chen HY, Huang TC, Ali M, Chang HY, Shieh TM, Shih YH, Wang KL, Huang YJ, Chung CP, Hsia SM. The Adipokine Visfatin Modulates Cancer Stem Cell Properties in Triple-Negative Breast Cancer. Biomedicines. 2023 Jan 20;11(2):297. doi: 10.3390/biomedicines11020297. PMID: 36830834; PMCID: PMC9953233.
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