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- GIBCO
- 120-17-100UG
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
充足
- 供应商：
GIBCO
- 英文名：
Human Wnt-1
- 规格：
100ug
Human Wnt-1 (100ug)
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Product Details |
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Size |
100 µg |
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Species |
Human |
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Published Species |
Non-human primate, Human, Mouse |
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Expression system |
E. coli |
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Amino acid sequence |
ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL |
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Molecular weight |
38.4 kDa |
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Class |
Recombinant |
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Type |
Protein |
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Purity |
98% by SDS-PAGE gel and HPLC analyses. |
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Endotoxin concentration |
<1 EU/µg |
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Activity |
The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2. |
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Conjugate |
Unconjugated |
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Form |
Lyophilized |
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Purification |
purified |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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Applications |
Tested Dilution |
Publications |
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Western Blot (WB) |
Assay-dependent |
- |
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ELISA (ELISA) |
Assay-dependent |
- |
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Functional Assay (Functional) |
Assay-dependent |
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In vitro Assay (IV) |
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12 Publications |
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Miscellaneous PubMed (Misc) |
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4 Publications |
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Product Specific Information
Recombinant Human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis
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文献和实验[1]Huang L, Desai R, Conrad DN, Leite NC, Akshinthala D, Lim CM, Gonzalez R, Muthuswamy LB, Gartner Z, Muthuswamy SK. Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Cell Stem Cell. 2021 Jun 3;28(6):1090-1104.e6. doi: 10.1016/j.stem.2021.03.022. Epub 2021 Apr 28. PMID: 33915081; PMCID: PMC8202734.
[2]Kumar Katakam S, Tria V, Sim WC, Yip GW, Molgora S, Karnavas T, Elghonaimy EA, Pelucchi P, Piscitelli E, Ibrahim SA, Zucchi I, Reinbold R, Greve B, Götte M. The heparan sulfate proteoglycan syndecan-1 regulates colon cancer stem cell function via a focal adhesion kinase-Wnt signaling axis. FEBS J. 2021 Jan;288(2):486-506. doi: 10.1111/febs.15356. Epub 2020 May 25. PMID: 32367652.
[3]Gu X, Mallipattu SK, Guo Y, Revelo MP, Pace J, Miller T, Gao X, Jain MK, Bialkowska AB, Yang VW, He JC, Mei C. The loss of Krüppel-like factor 15 in Foxd1+ stromal cells exacerbates kidney fibrosis. Kidney Int. 2017 Nov;92(5):1178-1193. doi: 10.1016/j.kint.2017.03.037. Epub 2017 Jun 24. PMID: 28651950; PMCID: PMC5651204.
[4]Zhang Y, Liu C, Duan X, Ren F, Li S, Jin Z, Wang Y, Feng Y, Liu Z, Chang Z. CREPT/RPRD1B, a recently identified novel protein highly expressed in tumors, enhances the β-catenin·TCF4 transcriptional activity in response to Wnt signaling. J Biol Chem. 2014 Aug 15;289(33):22589-22599. doi: 10.1074/jbc.M114.560979. Epub 2014 Jun 30. PMID: 24982424; PMCID: PMC4132767.
[5]Sundberg M, Bogetofte H, Lawson T, Jansson J, Smith G, Astradsson A, Moore M, Osborn T, Cooper O, Spealman R, Hallett P, Isacson O. Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons. Stem Cells. 2013 Aug;31(8):1548-62. doi: 10.1002/stem.1415. PMID: 23666606; PMCID: PMC3775937.
Mapping of the Wnt/-Catenin/TCF Response Elements in the Human Versican Promoter
activator β-catenin is the key mediator of the canonical Wnt signaling pathway. However, β-catenin does not itself bind DNA and thus functions via interaction with TCF/Lymphoid-enhancing factor (LEF) transcription factors. These proteins contain a high
: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U
【转帖】Top 100 proteomics publications
Top 100 proteomics publications ----From Ion Channel Media Group：ttp://www.ionchannelmedia.com/websites.php 1. Gavin et al. (2006) Proteome survey reveals modularity of the yeast cell machinery. Nature 2. Bonetta et al. (2006) Protein
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