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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 适应物种:
Human;Rat;Mouse;
- 保质期:
1年
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
Rabbit
- 应用范围:
WB;IHC
- 规格:
40μ/100ul/200ul
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文献和实验Measurement of Phosphoinositide 3-Kinase Activity
The term “PI 3-kinase” is now applied to a growing family of proteins that are able to convert phosphatidylinositol (PtdIns), PtdIns(4)P and PtdIns(4,5)P 2 into PtdIns(3)P, PtdIns(3,4)P 2 and PtdIns(3,4,5)P 3
Measurement of Phosphatidylinositol (PI) Hydrolysis in Activated T Lymphocytes
T-cell activation by antigen-receptor ligation results in rapid activation of phospholipase-Cγ1 (PLC). Activated PLC hydrolyzes membrane inositol phospholipids (PIP 2 ) generating two second messengers: inositol(1,4,5)tris phosphate (IP
of interest available; 2) formation of the antibody-antigen complex by adding specific antibody; 3) separation and detection of the immune complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Both polyclonal and monoclonal
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