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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 适应物种:
Human;Mouse;Rat
- 保质期:
1年
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
Rabbit
- 应用范围:
WB
- 规格:
40μ/100ul/200ul
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes
A simple and reliable method to measure proteoglycan synthesis by chondrocytes in culture is described. Confluent chondrocytes in 24-well plates are labeled for 24–72 h with 35 SO4 2− in the presence of stimulating agents. At the end
。 3.2 SMMP溶液:55 ml 2×SMM,40 ml 4×PAB,5 ml 10%(w/v)BSA,调pH值到7.0,过滤除菌。 3.3 2×SMM:25 ml 0.2 M的马来酸(顺丁烯二酸)钠盐,40 ml 0.1 N NaOH,调pH值到6.5,加入5 ml 1 M MgCl2,42.7 g 蔗糖,溶解定容至125 ml,过滤除菌。 3.4 4×PAB:牛肉膏6 g,酵母膏6 g,蛋白胨20 g,葡萄糖4 g,NaCl 14 g,K2HPO4 14.72 g,KH2PO4 5.28 g
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