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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
1D3
- 标记物:
Purified
- 规格:
100 μg
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文献和实验thresholds for antigen receptors and other surface receptors on B cells; also regulates B cell development, activation and differentiation CD19 antibody inhibits B cell activation from anti-immunoglobulin Coreceptor with CD21 Earliest B cell
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
policeman.4. Put the extract into an eppendorf and boil for 5 min., cool on ice.5. Load onto 10% SDS-PAGE. I usually load about 1/5 of the lysate from a 60 mm dish. This is about 100 μg protein for PC12 cells.6. After electrophoresis, electrotransfer
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