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- 详细信息
- 文献和实验
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- 克隆性:
15A7
- 标记物:
PE
- 规格:
50ug
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文献和实验Electroporation is a method for introducing DNA into cells by using a high-voltage electric field. This method is very simple and easily manipulated. We describe here a method for the modification of tumor cells with the Fas/Apo-1 (CD95) antigen
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Isolation and Propagation of Mouse Liver-Derived Dendritic Cells
, these lymphoid-derived DC can be generated in the absence of granulocyte macrophage colony stimulating factor (GM-CSF), and are CD8+ and Fas ligand+ (CD95L+ ). In the presence of GM-CSF, DC develop directly from myeloid committed precursors, which also give rise
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