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- BioGems
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- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
Psel.KO2.3
- 标记物：
PE
- 规格：
100 ug
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Intracellular Cytokine Staining Protocol
28 (2ug/ml soluble) 5hrMonensinJES5-16E3IL-12mouse PECmIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr)2hr/22hr-MonensinC17.8GM-CSFmouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble
,PE 50T/100T 1000/1500 L0114 Mouse anti-Human CD45RA mAb,Biotin 50T/100T 800/1200 L0115 Mouse anti-Human CD45RA mAb,Biotin 100ug/500ug
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