Recombinant Mouse IL-21, Tag F

An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources

The Transdirect insect cell is a newly developed in vitro translation system for mRNA templates, which utilizes an extract from cultured Spodoptera frugiperda 21 (Sf21) insect cells. An expression vector, pTD1, which includes a 5′

High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli

/L of functional protein. Herein, we report on the production in Escherichia coli and the purification of a recombinant mature streptavidin bearing a T7-tag. Optimization of critical parameters, including the glucose concentration, the pH and the time

Quantification of Mouse IL-6 and TNF- mRNA Using Xplore Assays

to measure both concentration of the free cytokine in solution and cytokine mRNA in the cytokine-expressing cells. Traditional methods of examining gene expression, such as as Northern blots, reverse transcriptase polymerase chain reaction (RT-PCR), and RPA