DiscoveryProbe™ TGF-beta/Smad

Multiple Cell Lines Using Quantum Dots

from a particular assay procedure, resulting in a significant reduction in the overall cost of a chemical compound library screen. By labeling live cells with Qtracker™ cell labeling kits and identifying cell proliferation using an Acumen Explorer� microplate

The Basics: RNase Control

decontamination solution such as Ambion's RNaseZap ™ (see below), or a mild solution of bleach or NaOH. Using a detergent solution like the kind used for soaking and cleaning glassware will not be as effective as these solutions. Detergent solutions may actually

Raf 蛋白 Ras 结合结构域的简并进化库合成以及利用片段互补法快速筛选二氢叶酸还原酶的快速折叠且稳定的克隆

类似物， 其抑制大肠杆菌 DHFR 的能力是抑制哺乳动物 DHFR 的 12000 倍）处理的大肠杆菌的生长依赖于重组 mDHFR 的表达 [ 35 ] 。这样，将两个目的蛋白分别与 DHFR 的两个片段融合并共表达，如果两个蛋白质之间有相互作用，则大肠杆菌可以在添加了甲氧苄氨嘧啶的基本培养基上生长 [31]。在一个最初的文库间筛选中，DHFRPCA 被用于从含有 6 X1010 个可能结合的序列的单独文库中鉴定出可以形成最优的形成亮氨酸拉链的异源二聚体序列。竞争实验（competition