Sec-O-Glucosylhamaudol-Sec-O-G

PCR引物设计和优化（PCR PRIMER DESIGN AND REACTION OPTIMISATION ）

that are possible , whether the temperature is reduced or not. Normally the denaturation time is 1 min at 94 o C : it is possible, for short template sequences, to reduce this to 30 sec or less . Increase in denaturation

Yale RNA prep

with white inserts. Transfer supernatant to new 15 ml c/f tubes(2). Add 800 l chloroform to each tube. Vortex 15 sec, incubate at room temperature for 3 min. Smeary preps will result if vortexing not long enough. Spin at 4°C, 4000 rpm

Worm PCR

for the template DNA and the primers you are using in the reaction: Melting step : 94°C for 30 sec (standard) Annealing step : ~5°C below melting pt. of the primers and usually held for between 30 seconds and 1 min. Extension step