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Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose

in outcomes. The procedure provided below has proven to be highly reproducible. It is particularly effective for the purification of m-calpain, although it is also useful for μ-calpain. Others have described the use of Blue Sepharose-CL-6B for the purification

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Heparin-Sepharose Precipitation and Immunoblotting To analyze for CTGF protein expression, conditioned media were collected and the heparin-binding proteins were precipitated by end-over-end mixing for 4 h at 4°C with heparin-Sepharose CL-6B beads

线虫蛋白提取--Making Protein Gel Samples from Worms

. Boiling will dissolve the adults, but the eggs will not be lysed. 6B. If you want to have embryos in your sample, put the worms in a 2 ml eppendorf tube. Add protein sample buffer to the worm suspension until there is a total of 1 ml in the tube