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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
/
- 库存:
现货库存
- 供应商:
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- 肿瘤类型:
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- 细胞类型:
/
- 品系:
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- 组织来源:
ATCC/DSMZ/ECACC
- 相关疾病:
/
- 物种来源:
人或动物
- 免疫类型:
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- 细胞形态:
/
- 是否是肿瘤细胞:
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- 器官来源:
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- 运输方式:
常温或干冰
- 年限:
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- 生长状态:
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- 规格:
T25
| 种属 | 人 |
| 组织来源 | 正常主动脉组织 |
| 传代比例 | 1:2传代 |
| 完全培养基配置 | 基础培养基500ml ;生长添加剂5ml ;胎牛血清25ml ;双抗5ml |
| 简介 | 主动脉内皮细胞(aortic endothelial cells)组成了主动脉内壁 ,并持续受到血流剪切应力的影响。内皮细胞在切应 力的作用下 ,分泌不同的内皮因子并进而影响血管收缩和生长。主动脉内皮细胞也调节细胞黏附分子的表达来控制和 精确调节炎症反应和组织纤维化。体外培养的原代主动脉内皮细胞可有效地帮助研究者研究内皮功能失调的机理 ,动 脉粥样化等疾病的发病机理以及发展新的治疗方法。 |
| 形态 | 上皮细胞样 ,多角形细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | 血小板-内皮细胞粘附分子(PECAM-1/CD31)免疫荧光鉴定 ,细胞纯度可达90%以上 ,不含有HIV-1、HBV、 HCV、支原体、细菌、酵母和真菌等。 |
Introduction The aim of this study was to identify patients with malignant hand lesions, establish the proportions of those that were metastases and review their clinical course. Methods A retrospective search of a prospective tumour database was carried out to identify all patients treated at our unit with hand metastases. Patient demographics were recorded including site of primary malignancy, region of the hand involved, management of their metastasis and clinical outcome. Results Overall, 149 patients were identified with a malignant tumour of the hand. Ten had a metastatic lesion. There were 3 women and 7 men with a median age of 68 years (range: 28-91 years) at presentation. All presented with non-mechanical hand pain while four had pain and swelling.
Gynaecol , 2021 , 41(6) : 977 - 980.as the patient outcome. According to recent evidence, it has been reported that they harness different immunological pathways to escape immunosurveillance and express aberrantly immunomodulatory agents or decreased levels of factors forming antigen presenting machinery (APM), suwtrquently followed by impaired antigen presentation and suppressed immune
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文献和实验/Gong G , Yin C , Huang Y , et al. A survey of influencing factors
of missed abortion during the two - child peak period[ J] . J Obstet
Gynaecol , 2021 , 41(6) : 977 - 980.
thx221 有没有哪位大侠做内皮细胞方面的信号转导的???帮忙给提供几个通路做参考!或者给我点相关的最新研究文献!我实在是找不到这方面的最新研究进展,大家帮个忙吧!先谢谢了!!! 编年张 加此QQ492207858 chuckdouble thx221 wrote: 有没有哪位大侠做内皮细胞方面的信号转导的???帮忙给提供几个通路做参考!或者给我点相关
hbsyliuyang rt 请不吝赐教 感谢! david_xmu 没什么太大的区别,另外内皮细胞在不同的培养基中培养可能会有形态上的区别 hbsyliuyang 谢谢这位达人! 拜 本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴 师兄微信号:shixiongcoming
正常人脐静脉原代内皮细胞培养一、实验试剂1、培养基: PriCells Medium + 10% FBS + 1% P/S + PriCells Supplem e nt2、冻存液: PriCells Medium + 20% FBS + 10% DMSO3、洗涤液: 1 × PBS (pH 7.4 ) + 1% P/S4、染色液: 0.4% Trypan Blue5、消化液: PriCells Isolation of Primary Cell Kit6、检测试剂:抗人
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