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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
/
- 库存:
现货库存
- 供应商:
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- 肿瘤类型:
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- 细胞类型:
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- 品系:
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- 组织来源:
ATCC/DSMZ/ECACC
- 相关疾病:
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- 物种来源:
人或动物
- 免疫类型:
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- 细胞形态:
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- 是否是肿瘤细胞:
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- 器官来源:
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- 运输方式:
常温或干冰
- 年限:
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- 生长状态:
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| 种属 | 人 |
| 别称 | LN-229+LUC |
| 组织来源 | 大脑 ;右额叶顶枕皮质 |
| 疾病 | 胶质母细胞瘤 |
| 传代比例/细胞消化 | 1:2传代,消化2- 3分钟 |
| 完全培养基配置 | DMEM培养基 ;5%胎牛血清 ; 1%双抗 |
| 简介 | LN- 229细胞系于1979年从一名右额叶枕部胶质母细胞瘤患者的细胞中建立。 |
| 形态 | 上皮细胞样 |
| 生长特征 | 贴壁生长 |
| 倍增时间 | ~31h |
| 致瘤性 | Yes, forms tumors in nude mice. |
| STR | Amelogenin: X CSF1PO: 12 D13S317: 10, 11 D16S539: 12 D5S818: 11, 12 D7S820: 8, 11 THO1: 9. 3 TPOX: 8vWA: 16 , 19 |
| 培养条件 | 气相 :空气 ,95% ;二氧化碳 ,5%。温度 :37摄氏度 ,培养箱湿度为70%-80%。 |
| 冻存条件 | 冻存液 :90% FBS , DMSO 10%, 或使用非程序冻存液 :官网货号JY-H040 |
| 备注 | 该细胞通过慢病毒转染的方式携带Luc基因 |
| 产品使用 | 仅限于科学研究 ,不可作为动物或人类疾病的治疗产品使用。 |
layer was interposed between the BM and the LPCM for 0.70±0.40mm (29.0±18.4%). In the pars plana region free of LPCM close to the ora serrata, the percentage of BM covered by the capillary layer was higher than in the pars plana region containing the LPCM (63.0±42.1% vs. 29.0±18.4%; pConclusions: The direct contact of BM with the LPCM in the pars plana in association with focal BM thickening at the LPCM end suggests an insertion of LPCM on the BM. Taking into account the biomechanical strength of BM, it may imply a functional unit of the LPCM with BM in the process of accommodation with a secondary movement of the posterior BM and tertiary thickening of the subfoveal choroidal space.
characterized. We provide a comprehensive generalized review of the TIlk that can inform GEP-NEN treatlknt strategies.Heat shock proteins (HSPs) are a group of proteins that promote protein folding, inhibit denaturation of cellular proteins, and maintain other proteins functional activities when cells are subjected to stress and/or high
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文献和实验/characterized. We provide a comprehensive generalized review of the TIlk that can inform GEP-NEN treatlknt strategies.Heat shock proteins (HSPs) are a group of proteins that promote protein folding, inhibit denaturation of cellular proteins, and maintain other proteins functional activities when cells are subjected to stress and/or high
【求助】请教荧光素酶报告基因检测NF-KB转录活性需要哪些试剂?
阿兰梦 之前没有做过,只是根据文献的简单描述依葫芦画瓢,但是毕竟是好几千的试剂,生怕弄错了 临床医生一下子做科研,确实很多地方不懂,很艰难 目的是想用双荧光素酶报告基因的方法检测药物内皮细胞的NF-KB的转录活性影响,需要买pNF-kB-Luc 报告质粒,碧云天有;然后需要双荧光素酶报告基因检测系统,promega有;另外还需要一个内参,文献是β-半乳糖苷酶 问题是: 1、不知道买哪一个对: http://www
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