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- 2025年12月23日
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文献和实验Preparation of Fluorescent DNA Probe from mRNA: Yeast
Amersham #55022 Microcon-30 filter 3 Amicon #42410 TE (pH 7.4) ~5 mL polyA DNA or RNA (10 mg/mL in TE) 1.0 uL 20X SSC 3.0 uL
Non-fluorescent protein/RNA double-labeling
the embryos with 3% hydrogen peroxide in methanol for 15 min. Rinse well in methanol. 3. Wash 3 X 30 minutes with PBTH (filtered 1 X PBS, 0.1% Tween-20, 50 ug/ml heparin, and 250 ug/ml tRNA). You can also include 1% BSA providing you know it is ultra pure
Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kDa protein absorbs blue light from a photoprotein that is activated by calcium and emits green
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