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文献和实验lectins labeled with particles of colloidal gold and used them in scanning electron mrcroscopy (10 ) Subsequently, gold-labeled lectins were applied in transmission electron microscopy to study various aspects of cell surface expression
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
the focus systematically up and down in 1 um steps and triggering the ablation laser. The expanded, aligned, and convergence adjusted beam should ablate maximally at the image focus and weakly or not at all 1 um above or below focus (fig 5). 13)
一、材料和试剂 1. 抗体 (1)Ratanti-mouseCXCR5-biotin(BP Pharmingen 551960) (2)Goatanti-ratIgG-biotin(BP Pharmingen 554014) (3)Straptavidin-PECy7(B PPharmingen 557598) (4)Ratanti-mouseB220-pacificblue450(eBioscience 48-0452-82)
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