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- 2025年07月08日
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
. The transformation efficiency is not important if one already has a pure plasmid with the insert and just want to transform to grow more of the plasmid. Transformed bacteria are plated on a culture plate that has antibiotics. After 15-20 hours, colonies
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, H. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell 139:610‐622. Hudson, J.R., Jr., Dawson, E.P
were eluted with two 3-ml portions of elution buffer (Pierce kit); diluted to 13 ml with TBS; dialyzed at 4ºC against two changes of TBS and three changes of PBS (0.15 M NaCl,5 mM NaH2PO4,pH adjusted to 7.0 with NaOH); and stored at –20ºC.Protein concentration
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