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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
DETECTION OF ß-GALACTOSIDASE The E. coli lacZ gene encoding ß-galactosidase (ß-gal) is the classical
Magnetic Fluids Their Applications to Medicine
of which is illustrated in figure 1. Magnetic particles are coated with a reagent which selectively attaches to the target cells when added to the cell suspension. After an incubation period the target cells are isolated by use of a magnetic field. This method offers
A quick method to isolate plant genomic DNA.
well. Keep the solution on ice for i 30 min, (precipitation of proteins). Spin down the precipitations at 7000 rpm, 5 min. Transfer 300 ul of the supernatant to a new eppendorf tube. Add 900 ul NaI (GeneClean II), + 20 ul "glass milk" (silica particles
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