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- 百欧泰(biotyscience)
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- 2025年07月15日
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文献和实验SSR GEL and Silver Staining Protocol
are increased by 50%. 4. Rinse the gel 3 times for 2 minutes each with distilled water with gentle agitation. 5. Add staining solution to gel and gently agitate for 30 minutes, decant and precipitate silver. The silver in the used staining solution
Inverse PCR & Cycle Sequencing
I. Quick Fly Genomic DNA Prep Standard fly mini prep (30 flies) resuspended in 150 ul TE 1) Collect 30 anesthetized flies in eppendorf tube and freeze at -80°. 2) Grind flies in 200 µl Buffer
SSR GEL and Silver Staining Protocol
, Gallard-Schleisinger Ind., at -20℃.) 3. Warm and stir the mixture in a beaker of warm water until all the urea is dissolved. Add 1.25 g of amberlite resin and stir 5 min. Filter through a 0.2 uM filter and degas at 25 mg Hg for 5 min. Transfer to graduate
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